Shang Y, Li Y, Wang Z, et al. - Researchers conducted the study for analyzing the pathogenicity and immunogenicity of human corneal stromal lenticules from small incision lenticule extraction (SMILE). Serological testing to rule out infectious diseases was performed before sample collection. In total, 132 lenticules from 93 patients were assigned randomly to the fresh group, −78 °C anhydrous glycerol preservation group (glycerol group), and 0.1% sodium dodecyl sulfate decellularization group (SDS group) in pairs and detected by immunohistochemistry, Western blot, transmission electron microscopy, transmittance, and nanoindentation. The risk of infection is minimal, but the reuse of fresh human corneal stromal lenticules from SMILE poses a risk of rejection. The preservation of anhydrous glycerol at −78 °C is an optimal method for reducing antigens without damaging the lenticules structure and function.