Birkhoff WAJ, et al. - Researchers, for the first time, investigated the performance of the retinal function imager to analyZe retinal oximetry measurements and to quantify capillary maps in sickle cell disease (SCD) patients (n = 8) and matched healthy controls (n = 8). By the retinal function imager, oximetry pictures and non-invasive capillary perfusion maps have been obtained. No significant difference was found in vessel density, saturation, and deoxygenated Hb maps between SCD patients and matched controls in all anatomical quadrants and annular zones. Oxygenated Hb levels in patients with SCD were significantly lower than in matched controls. The research proved the feasibility of multispectral imaging in both SCD patients and matched volunteers to assess retinal changes in oxygenation. The obtained data indicate that functional abnormalities can be observed with abnormal oximetry measurements in patients with SCD before any structural microvascular changes occur in the central retina.