Feng F, et al. - The present study was undertaken to examine the clinicopathological features and prognosis of gastric cancer patients with well, moderately, and poorly differentiation status by analyzing data of 3,090 gastric cancer patients treated with radical D2 gastrectomy from September 2008 to March 2015. It was noted that there were 370 (12.0%) well differentiated tumors, 836 (27.0%) moderately differentiated tumors and 1,884 (61.0%) poorly differentiated tumors. The study results showed that well and moderately differentiation status were correlated with older age, male gender, smaller tumor, shallower invasion, less lymph node involvement, and earlier tumor stage. Findings revealed that well, moderately and poorly differentiation status was significantly related to clinicopathological features of gastric cancer patients, but not to the prognosis of gastric cancer patients.