Qin H, et al. - Researchers assessed the role of MYBL2 protein expression in esophageal squamous-cell carcinoma (ESCC). Using immunohistochemistry, they analyzed tissue samples of ESCC to determine the expression of MYBL2 and Ki67. They used a CCK8 assay, 5-ethynyl- 2'-deoxyuridine–retention assay, flow-cytometry analysis, real-time quantitative PCR, Western blot, and a xenograft model of ESCC cells in nude mice to determine the impact of MYBL2 on cell proliferation and DNA replication. Poor patient outcome was observed in relation to MYBL2 expression in ESCC tissue. The worst prognosis for overall survival was evident in MYBL2-positive patients who had high Ki67 expression in ESCC tissue. By regulating cell-cycle at the S and G2/M phase, tumor proliferation in ESCC cells was caused by MYBL2 overexpression. This implies that, in ESCC patients, MYBL2 could be a new prognostic biomarker.