Amoah LA, Donu D, Abuaku B, et al. - In this cross-sectional study enrolling 729 people aged 85 years old and below from seven closely linked communities, experts quantified the burden of Plasmodium malaria infection in seven communities in the Eastern Region of Ghana. Nearly 96.6% of the individuals were distinguished as afebrile. No important variations were noted in falciparum and non-falciparum parasite carriage at the community level, nevertheless, young adults aged between 15 and 19 years had the highest prevalence of P. falciparum and Plasmodium malariae parasite carriage while the greatest level of Plasmodium ovale carriage was seen in children aged between 5 and 9 years. The high rate of misidentification of non-falciparum parasites and the total absence of identification of P. ovale through microscopy implies that more sensitive malaria diagnostic tools involving molecular assays are needed to accurately ascertain the predominance of carriers of non-falciparum parasites and low-density Plasmodium falciparum infections, particularly during national surveillance exercises. Moreover, malaria control interventions targeting the non-falciparum species P. malariae and P. ovale parasites are required.