Cloots K, Uranw S, Ostyn B, et al. - Researchers explored the validity of the direct agglutination test (DAT) as a marker of infection, because of future surveillance systems. In this study, a total of 6,609 participants were analyzed between October 2017 and December 2016. Age group-specific and cluster-specific seroprevalences in 2016 were compared with those in 2006, applying χ2 tests, with a specific focus on the comparison of seroprevalences in children born between 1996 and 2005, and those born between 2006 and 2015. They fitted a Poisson model controlling for age, sex, and cluster to determine the overall adjusted risk ratio for being seropositive in 2016 compared with 2006. The results of this study revealed that, in Nepal, the transmission of L donovani has decreased significantly between 2006 and 2016, coinciding with the elimination programme. Direct agglutination test appears beneficial for monitoring of L donovani transmission.