Pedrotti E, Bonacci E, De Rossi A, et al. - This study was undertaken to determine if type 1 big-bubble (BB) formation is affected by the sequence of incisions created with the Victus femtosecond laser (FSL) enabled with software version 3.4 (SV 3.4) during deep anterior lamellar keratoplasty (DALK). Consecutive FSL-assisted DALK BB procedures were conducted on 20 human donor corneas: 10 shaped by tunnel incision followed by lamellar incision (tunnel-lamellar group, TL) and 10 in the reverse order (lamellar-tunnel group, LT). In order to obtain a type 1 BB by air injection, shaping the tunnel before rather than after a lamellar incision may be more efficient.