Li H, Dong M, Zhou Q, et al. - In the present study, the researchers sought to characterize the corneal calcification of acellular porcine corneal stroma (APCS) after lamellar keratoplasty (LKP) and identify risk factors. Two cases of APCS calcification were assessed by slit‐lamp photography and anterior segment optical coherence tomography (AS‐OCT). von Kossa staining and scanning electron microscope/energy‐dispersive spectrometry (SEM/EDS) were conducted on pathologic tissue. Findings suggested that APCS calcification occurs in the superficial corneal stroma about 1 month following LKP. The application of AS‐OCT, von Kossa staining and SEM/EDS provides a basis for corneal calcification's clinical and pathological diagnosis. High phosphorus content and downregulated calcium metabolism-related proteins in APCS were mainly the associated risk factors. Corneal calcification may be encouraged by postoperative epithelial defects, inflammation and the use of phosphorous eye drops.