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Comparison of one commercial and two in-house Taqman multiplex real-time PCR assays for detection of enteropathogenic, enterotoxigenic, and enteroaggregative Escherichia coli

Tropical Medicine & International Health Sep 19, 2017

Hahn A, et al. - A comparison of commercial PCR kit and two in-house assays without using a gold standard was performed to realize the sensitivity and specificity of each assay. Observations revealed commercial and in-house assays as comparable regarding results, this suggested in-house tests as safe while affording considerable savings. This made them a valuable alternative for surveillance testing in resource-limited tropical areas.
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