Curtis KA, et al. - Considering a possible utility of the incidence assay analytes, which are remarkably predictive of time since seroconversion, for predicting recent transmission events during HIV outbreaks and informing prevention strategies, researchers tested plasma samples (n = 154) from a recent HIV-1 outbreak in a rural community in Indiana with the customized HIV-1 Multiplex assay, based on the Bio-Rad Bio-Plex platform, which measures antibody response to HIV envelope antigens, gp120, gp160, and gp41. For predicting seroconversion within 30, 60, and 90 days, sensitivity/specificity of the HIV-1 Multiplex assay was 90.5%/95.4%, 94.1%/90%, and 89.4%/82.9%, respectively, based on a training data set. Findings suggest the potential utility of the HIV-1 multiplex assay for identifying and monitoring trends in recent infection during an epidemic to assess the effectiveness of programmatic or treatment interventions.